HYDROGEL BASED WOUND DRESSING USING BIOPOLYMER WITH IMPROVED WOUND HEALING ACTIVITY

摘要

The aim of the present investigation was to prepare and evaluate Hydrogel based wound dressing of functional bio polymers for wound care application. Topical drug administration is a localized drug delivery system anywhere in the body through ophthalmic, rectal, vaginal and skin as topical routes. Skin is one of the most readily accessible organs on human body for topical administration and is main route of topical drug delivery system. Optimization of final formulation was performed by applying 3 full factorial design. Hydrogel based wound dressing containing povidone-iodine was prepared using solvent casting method. Drug-excipients incompatibility study was carried out using Fourier Transform Infrared Spectroscopy (FTIR). The analytical method was developed using UV-visible spectrophotometer. Hydrogel based wound dressings were evaluated for thickness, folding endurance, % elongation, tensile strength, drug content and weight variation. The Anti-microbial study was performed using agar diffusion method. In-vitro drug release study was performed by Franz diffusion cell. In-vivo wound healing study was performed using rat model. The stability study was performed at accelerated conditions as per ICH guidelines. Drug-drug and drug-excipient were found to be compatible with each other. The developed analytical method was compatible for quantitative analysis of povidone-iodine. The hydrogel based wound dressing was successfully prepared with povidone-iodine using sodium alginate, propylene glycol and aloe vera solution containing heparin (0.1 % w/v). The thickness,' folding endurance, % elongation, drug content and tensile strength of optimized batch was found to be optimum. Hydrogel based wound dressing containing povidone-iodine shows most promising antimicrobial activity and it is as effective as povidone-iodine marketed formulation. The in-vitro drug release study shows sustained release up to 10 hours. In-vivo wound healing study indicates 99.26 % part of the rat wound was healed in 5 days. The stability study shows povidone-iodine containing hydrogel based wound dressing was stable at accelerated condition.