HYDROGEL SCAFFOLD COMPRISING ALGINATE-CHITOSAN LAYERED COMPOSITE, BLENDED GEL MATRIX WITH EMBEDDED VOIDS AND TUNABLE DIMENSIONS

摘要

A hydrogel scaffold comprising scaffold of rigidly cross-linked biopolymer having tunable structure of induced multilayer voids of desired macroporosity, elasticity and strength is provided together with methods of preparation of the same. The methods implemented in the present invention for the preparation of scaffold are selected from single phase blending of any one or more biopolymers including (i) blending of gelatin and alginate, and (ii) coating chitosan over alginate wherein a fluidic device was utilized to introduce self-aligned series of bubbles that left microvoids in the final structure said voids formed of surfactant sustained bubble induced in said biopolymer, which scaffold is advantageously suitable for end use and application such as delivering biological agents to the host tissue, providing a three-dimensional support for matrix formation.