MULTIPLEX REALTIME PCR KIT FOR DETECTING DRUG-RESISTANT STRAINS OF M. TUBERCULOSIS

摘要

A system and process is provided for detecting M. tuberculosis strains or mutants thereof, particularly those strains resistant to first line anti-tuberculosis drugs, second line anti-tuberculosis drugs or a combination of both for e.g. isoniazid, rifampin, fluoroquinolone / aminoglycoside, kanamycin or pyrazinamide or any combination thereof in a biological sample, said system comprising: isolating and further amplifying nucleic acids under hybridizing conditions with specific sequences of primers and fluorophore-attached-probes that target portions of the katG, inhA, gyrA, rpoB, pncA or rrs or any combinations thereof and real-time detection of the amplified nucleic acids to determine the presence or absence of M. tuberculosis strains or mutants thereof. To be published with Figure 1