New selective optical sensor for determination of protein concentrations, in particular human albumin, using polymers, in-printed by molecular typing method with dual refraction of liquid crystals

摘要

the object of the application is a new chemical sensor for the determination of proteins, especially albumin human serum (hsa)with osadzanu0105 by elektropolimeryzacji layer rozpoznaju0105cu0105 as conducting polymer derivatives bisbitiofenu, wdrukowanego molekularnie by proteins.the notification also includes the use of a liquid crystal dwu00f3ju0142omnou015bci phenomenon, in combination with the use of rozpoznaju0105cej described above.for selective detection and / or marking hsa in test samples.the application is characterized by the fact that the semikowalencyjnego wdrukowania molecular proteins, including of hsa, as functional monomers used acid 2,2 '- bitiofeno - 5 - acid and p - bis (2,2 '- bitien - 5 - yl) metyloalaninu0119 and as a monomer sieciuju0105cy apply 5,5', 5 '- tris (2,2' - bitienylometan)].to elektropolimeryzacji apply solution solvent mixtures, preferably acetonitrile and zbuforowanego normal saline solution containing protein (hsa) marked, and 5,5 ',5 '- tris (2,2' - bitienylometan)] and basic electrolyte, chlorate (vii) tetrabutylammonium.used to elektropolimeryzacji potencjodynamicznej potential is linearly changed in a cyclical manner in the range from 0 to 1.50 v, relative to the ag / agcl, at a constant rate in the range of 5 mv / s to 5 v / s.the polymer is deposited on the przetworniku signal chemical / (bus electrode from a group of fixed electrodes working) selected conductors and pu00f3u0142przewodzu0105cych transparent for light uv - vispreferably a glass plate with napylonu0105 pu00f3u0142przeu017aroczystu0105 gold layer thickness of ~ 50 nm.a transducer signal recognition chemical analytical signal in the described chemoczujniku applied thin layer liquid crystal with a high refractive index.this notification also involves the application of chemoczujnika or individual elements thereof, for selective detection and / or determination of proteins, hsa favourably.