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Gelatin hydrogel matrices, method for producing them and their applications

机译:明胶水凝胶基质,其制备方法及其应用

摘要

the subject of hydrou017celowe gelatin matrices are characterized by this, that contain synthetic substrate in the form of ortho - nitrofenylo - i - d - galaktopiranozydu (onpg) in a concentration from 80 to 20 mmol dm - - 3.the application also includes a way to produce gelatin matrices hydrou017celowych, which is that the aqueous solution of ph from 7.2 to gelatine 80 and concentration from 5 to 15% w / v containing ortho - nitrofenylo - i - d - galaktopiranozyd (onpg) in a concentration from 80 to 20 mmol dm - 3 - undergo cross-linking reaction in the presence of an aqueous solution transglutaminazy concentration from 0.1 to 2.0% w / v as a factor sieciuju0105cego during 0,25 h to 24 h and temperature from 277 to 293 k.the application also relates to application of gelatin matrices hydrou017celowych to manufacture colored test detection of beta galactosidase, in particular in aqueous solutions.
机译:水溶性明胶基质的特征在于,它含有浓度为80至20 mmol dm-3的邻硝基硝基苯并i-d-galaktopiranozydu(onpg)形式的合成底物。一种生产明胶基质的方法,即ph的水溶液从7.2到明胶80,浓度从5%到15%w / v,其中含有一定浓度的邻位-硝化烯基-i-d-galaktopiranozyd(onpg)从80到20 mmol dm-3-在浓度为0.1-2.0%w / v的水溶液中以谷氨酸转氨酶的形式在0,25 h到24 h的温度和277°C的温度下进行交联反应本发明还涉及应用明胶基质水合凝胶制造β-半乳糖苷酶的有色测试检测方法,特别是在水溶液中。

著录项

  • 公开/公告号PL421412A1

    专利类型

  • 公开/公告日2018-01-15

    原文格式PDF

  • 申请/专利权人 POLITECHNIKA WROCŁAWSKA;

    申请/专利号PL20170421412

  • 发明设计人 KAROLINA LABUS;

    申请日2017-04-26

  • 分类号C08J3/075;C12Q1/34;

  • 国家 PL

  • 入库时间 2022-08-21 12:50:20

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