Production of steroid 11-alpha hydroxylated by biotransformation with recombinant bacteria (Machine-translation by Google Translate, not legally binding)

摘要

Production of steroids 11 α hydroxylated by biotransformation with recombinant bacteria. The present invention provides a method for producing steroid compounds 11 α hydroxylated or derivatives thereof by fermentation of natural sterol or by biotransformation of different steroid synthons with recombinant bacteria, preferably mycobacterium smegmatis or corynebacterium glutamicum. Said recombinant bacteria carry a plasmid comprising a synthetic operon containing the genes cyp509cl2 and rocpr1 of rhizopus oryzae, which code for cytochrome cyp509c12 with activity 11 α -hydroxylase and cytochrome reductase (rocpr1) necessary for cytochrome activity. By means of the method described in the present invention, 11 α can be produced, but without limitation; oh-add or 11 α -oh-ad from natural sterols such as cholesterol or phytosterols, or 11 α -oh-prog, 11 α -oh-doc, 11 α -oh-test, 11 α -oh-dhea, 11 α -oh-ad and/or 11 α -oh-add from their corresponding non-hydroxylated synthons. (Machine-translation by Google Translate, not legally binding)