universal method for determining the threshold values ​​cycle real-time PCR

摘要

A method for determining a threshold value of Ct cycle in a growth curve a growth process, the method comprising: - receiving a dataset representing a growth curve, including the data set a plurality of data points , each data point a pair of coordinate values ​​of a cycle number and a signal strength of growth procedure in the cycle number; - calculating a function that approximates the data set; - determine a baseline of the growth curve, with the linear reference line; - calculating, with a computer system, a first point of the function in which a maximum occurs in a second derivative of the function; - determining with the computer system, a tangent line that is tangent to the function at the first point; - calculating, with the computer system, an intersection point of the tangent line and the reference line being the cycle number of the intersection point of the threshold value Ct cycle.