Mice derived from ES cells from injection of a diploid host embryo

摘要

A mouse embryo, comprising a cell that has in its genome: a) a site-specific recombinase gene functionally linked to a Nanog promoter that expresses the site-specific recombinase gene in a cell of the internal cell mass ( ICM) but not of the troofectoderm; (b) a gene encoding a toxin, functionally linked to a promoter, in which the toxin gene is present in a locus that allows expression; and (c) a nucleic acid sequence that prevents expression of the gene encoding the toxin, in which the nucleic acid sequence is located between the promoter of the gene encoding the toxin and the coding sequence of the gene encoding the toxin and it is flanked on both sides by recombinase recognition sites so that in the presence of the site-specific recombinase the nucleotide sequence is removed and the promoter for the gene encoding the toxin directs the transcription of the gene encoding the toxin.