Method of detecting chemicals in samples can be collected material from a subject, in particular, to detect embryotoxic factors

摘要

A procedure to detect in vitro embryotoxic factors in samples collected from a Human subject or an animal,That includes the following steps: preparing a sample of a substanceThe sample of a substance comprising preferably Organic Fluids and / or parts of tissue from a donor organism; and Associate with the element D a sample of a substanceAnd adequate control to verify the presence of at least one Active Agent in the sample of a substance; and to verify the presence or absence of said Active Agent Based on a change in theIn this control element.This control Element comprising at least one living organism has the capacity to Feed Itself autonomously through Temporary bodies associated with the living organismCapable of supplying Nutrients to the latter without taking these Nutrients from an External Environment.Wherein the verification phase of the presence or absence of the active Agent is designed to verify the absence or presence of embryotoxic factors (TEFS) and includesFollowing sub Stages: - maintain an environmental Interaction, preferably in containers of verification of a Culture PlateBetween embryos and the sample of a substance in a defined Order of Interaction varies from twelve hours or, preferably, multiple of 12 hours.Up to a maximum of 48 hours; - have a number of embryos died after this period of Interaction has been attributed to the sample; and a level of toxicity of a substanceThat is proportionate and quantitatively correlated with a present or a quantitative level of Chemical activity of these embryotoxic factors (TEFS).