A recombinant chimeric dengue virus rDEN3 / 4delta 30 (ME), rDEN2 / 4delta30 (ME) or rDEN1 / 4delta30 (ME) that contains a deletion of 30 nucleotides (delta30) in a section of the 3 'non-translated region of the genome of type 4 dengue, wherein said 30 nucleotide deletion corresponds to the stem-loop structure of TL2

摘要

A recombinant chimeric dengue virus comprising a nucleic acid chimera comprising a first nucleotide sequence encoding structural protein C of dengue virus type 4 and structural proteins M and E of dengue virus type 1, of virus of Dengue type 2 or dengue virus type 3, wherein the region of dengue virus type 4 encoding structural proteins M and E is replaced by the region encoding said structural proteins M and E dengue virus type 1, dengue virus type 2 or dengue virus type 3, and a second nucleotide sequence encoding non-structural proteins of dengue virus type 4 and the 3 'non-translated region of the dengue genome type 4 containing a 30 nucleotide deletion that corresponds to the stem-loop structure of TL2 between nucleotides 10478-10507 of the dengue genome type 4.