REENGINEERING MRNA PRIMARY STRUCTURE FOR ENHANCED PROTEIN PRODUCTION

摘要

Described herein are rules to modify natural mRNAs or to engineer syntheticmRNAs to increase their translationefficiencies. These rules describe modifications to mRNA coding and 3' UTRsequences intended to enhance protein synthesis by:1) decreasing ribosomal diversion via AUG or non-canonical initiation codonsin coding sequences, and/or 2) by evading miRNA-mediated down-regulation by eliminating one or more miRNA binding sites incoding sequences.