NON-OPTICAL LABEL-FREE BIOMOLECULAR DETECTION AT ELECTRIALLY DISPLACED LIQUID INTERFACES USING INTERFACIAL ELECTROKINETIC TRANSDUCTION (IET)

摘要

An embodiment in accordance with the present invention is directed to a non-optical, label-free microfluidic biosensor utilizing an electrical liquid interface between two co-flowing liquids - one with a higher conductivity and one with a higher dielectric constant. The analyte-of-interest is in one solution while the receptor is in the adjacent stream. The electric interface acts as a substrate, when an alternating current electric field is applied perpendicularly across the interface, liquid displacement occurs which is frequency dependent. When a reaction occurs at the interface, it alters the electrical properties of the electrical interface, altering the frequency dependent liquid motion, which is then monitored by impedance spectroscopy downstream.