CONSTRUCTION AND APPLICATION OF LENTIVIRAL VECTOR FOR SPECIFICALLY INHIBITING HUMAN MIRNA-424 EXPRESSION

摘要

A construction and application of a lentiviral vector for specifically inhibiting human miRNA-424 expression. The lentiviral vector comprises a fundamental sequence, a resistance gene sequence, a multiple cloning site sequence, a promoter sequence, and an oligonucleotide sequence targeting miR-424 of a pLKO.1-puro expression vector. A multiple cloning site comprises an Age I enzyme cutting site and an EcoR I enzyme cutting site; the oligonucleotide sequence targeting miR-424 is inserted into the multiple cloning site in a forward direction. A pLKO-Tud-424 lentiviral expression vector has the advantages of high transfection efficiency, a small amount required, and continuous, efficient, stable and specific inhibition in human miRNA-424 expression, and may serve as a powerful tool for preparing drugs for treating diseases related to abnormal miRNA-424 expression.