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LARGE SCALE PEI-MEDIATED PLASMID TRANSFECTION
LARGE SCALE PEI-MEDIATED PLASMID TRANSFECTION
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机译:大规模裴介导的质粒转移
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摘要
We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.
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