EXTRACHROMOSOMAL SWITCHING AUXOTROPHIES PROGRESSIVELY BY INTEGRATION (ESWAP-IN) FOR ASSEMBLY OF DNA SEQUENCES IN YEAST

摘要

Compositions, methods and kits are provided. The compositions, methods and kits are for assembly of series of DNA segments in yeast using homologous recombination. The assembled DNA segments are maintained episomally. Yeast made using the methods are included, as are methods of using the yeast to express proteins, and for screening test agents that can affect yeast that are modified to include the assembled DNA segments.