DEVICES AND METHODS OF TISSUE VISUALIZATION FOR USE IN LAPAROSCOPIC, ROBOT-ASSISTED LAPAROSCOPIC, AND OPEN PROCEDURES

摘要

Taught herein is a new contact-based optical imaging technology, en-face differential optical topography (en-face DOT), which performs real-time visualization of subsurface tissue heterogeneity within a depth up to 3mm and over a 9.5mm diameter FOV with a modest mm-level lateral resolution. An embodiment of the probe fits in a 12mm port and houses at its maximum 128 copper-coated 750μηι fibers that form radially alternating illumination (70 fibers) and detection (58 fibers) channels. By simultaneously illuminating the 70 source channels of the laparoscopic probe that is in contact with a scattering medium and concurrently measuring the light diffusely propagated to the 58 detector channels, the presence of near-surface optical heterogeneities can be resolved in an en-face 9.5mm field-of-view in real-time. Visualization of subsurface margin of strong attenuation contrast at a depth up to 3 mm is demonstrated at one wavelength at a frame rate of 1.3 Hz.