RAPID ENZYMATIC HYDROLYSIS OF SUBSTRATES FOR PRODUCTION OF FERMENTABLE SUGARS

摘要

The present disclosure provides a process for rapid hydrolysis of a substrate the process comprises the steps of obtaining a substrate slurry; contacting the 10 substrate slurry with at least one enzyme to obtain a soluble fraction and an insoluble fraction; separating the soluble fraction from the insoluble fraction; recovering the at least one enzyme from the soluble fraction to obtain recovered enzymes; and treating the insoluble fraction with the recovered enzymes to achieve at least 90% hydrolysis of the substrate in a time period in the range of 2 - 5 hours.