METHOD FOR MONITORING XENOTRANSPLANTATION IMMUNE REJECTION BY USING PORCINE SPECIFIC FREE DNA, AND PRIMERS

摘要

A method for monitoring xenotransplantation immune rejection by using porcine specific free DNA, and primers, wherein the method is used for monitoring xenotransplantation immune rejection in a recipient subject using pig as donor. The method includes: a recipient plasma DNA sample is subject to quantitative fluorescence PCR amplification by using primers having specific amplification for porcine specific free DNA, so as to quantify the amount of porcine specific free DNA in the plasma of the recipient. Quantifying porcine specific free DNA in plasma by using quantitative fluorescence PCR amplification has advantages such as non-invasiveness, high specificity, high sensitivity, simplicity and convenience, low detection cost and capability for continuous monitoring, thus having a broad prospect for market applications.