EXTRACELLULAR RATIO-TYPE OXYGEN SENSING PROBE AND PREPARATION METHOD AND USE THEREOF

摘要

Provided in the present invention are a ratio-type oxygen sensing probe and a preparation method and a use thereof. The ratio-type oxygen sensing probe comprises a carrier and an oxygen-sensitive compound wrapped in the carrier. The carrier consists of an amphiphilic graft polymer and an aggregation-induced luminophore attached to the amphiphilic graft polymer. The ratio-type oxygen sensing probe of the present invention can measure the conditions of oxygen consumption of Escherichia coli and GM12878 human B lymphocyte under different cell concentrations, and in the meantime, the probe can measure in situ bacteria of at least 100 cfu/mL within 7 hours, and can also be used for drug screening, and has no cytotoxicity and is safe and reliable. In addition, since the PtTFPP probe micelle shows a extremely high quantum efficiency and the oxygen sensing probe can measure the oxygen content in an aqueous solution, the probe can be widely applied to some commercial instruments like a microplate reader, providing assistance to the high through-put measurement, disease diagnosis and metabolism research.