ENGINEERED YEAST AS A METHOD FOR BIOREMEDIATION OF METALS

摘要

Metal bioremediation and metal mining strategies can include compositions and methods as follows: (1) A composition and a method for treating water for metal remediation or mining can include a cell and a first oligomer of a metal binding protein expressed on a surface of the cell via a linker, where the linker is tethered to the first oligomer of the metal binding protein and to a surface of the cell, the metal binding protein has specificity for a metal; and the first oligomer of the metal binding protein expressed on the surface of the cell is capable of aggregating with a second oligomer of the metal binding protein in the water upon binding a metal. (2) A composition and a method for treating water for metal remediation or mining can include a cell expressing a membrane metal transporter, where the membrane metal transporter has specificity for a metal, a vacuole transporter; and a metal sequestration protein. (3) A composition and a method for treating water for metal remediation or mining can include a cell including a knocked-out enzyme required in sulfate-assimilation pathway, where the cell has specificity for reactions against a metal.