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COORDINATED COMPOSITION GRADIENT AND TEMPERATURE GRADIENT LIQUID CHROMATOGRAPHY
COORDINATED COMPOSITION GRADIENT AND TEMPERATURE GRADIENT LIQUID CHROMATOGRAPHY
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机译:成分梯度和温度梯度液相色谱的协调
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摘要
A method of performing a chromatographic separation includes generating a spatial temperature gradient along a length of a chromatographic column in a liquid chromatography system. A sample is injected into a flow of a mobile phase to the column and a flow of a mobile phase having a composition gradient is provided to the column after the sample is received at the column. The spatial temperature gradient is moved along the length of the column from the column inlet to the column outlet during the time that the composition gradient traverses the column. This coordination of the composition gradient with the movement of the spatial thermal gradient yields a significant increase in peak capacity per unit time compared with conventional separation techniques performed in a conventional isothermal column environment.
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