IMPROVED LIBRARY PREPARATION AND USE THEREOF FOR SEQUENCING-BASED ERROR CORRECTION AND/OR VARIANT IDENTIFICATION

摘要

Aspects of the invention include methods for preparing sequencing libraries, performing sequencing procedures that can correct for process-related errors, and identifying rare variants that are or may be indicative of cancer, using loop-shaped adaptors ligated at both ends of a double stranded DNA and linearization of the obtained circular molecule by cleaving one of the loop-shaped adaptors. The linear ssDNA obtained comprises both the forward and the reverse complement strand.