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P450 Codon optimized cyclosporine specific P450 hydroxylase and whole cell biotransformation of cyclosporine in E. coli using thereof

机译：P450密码子优化的环孢菌素特异性P450羟化酶及其在大肠杆菌中环孢素的全细胞生物转化

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摘要

The present invention relates to; (CsA cyclosporine A) specific P450 hydroxide enzyme (hydroxylase) and cyclosporine bioconversion method coli-based optimization of using the same, the present invention is codon is shown in SEQ ID NO: 1 optimizes the invention codon optimized cyclosporin A It provides a cyclosporin specific gene hydroxide. The present invention is a recombinant vector comprising the hydroxide of the codon optimized gene; And a ferredoxin (ferredoxin) gene and the ferredoxin -NADP ⁺ reductase (ferredoxin-NADP ⁺ reductase) provides a recombinant microorganism transformed with a recombinant expression vector containing the gene. Using the recombinant microorganisms, cost-developed cell bioconversion system, the system scales of the hair growth promoter later-effective ago to produce CsA-4-OH is expected to be applied effectively to up production.

机译：本发明涉及; （CsA环孢素A）特异性P450氢氧化酶（羟化酶）和环孢素生物转化方法基于大肠杆菌的优化使用相同，本发明的密码子如SEQ ID NO：1所示，是本发明的密码子优化的环孢菌素A，它提供了环孢菌素特异性氢氧化物基因。本发明是一种重组载体，其包含密码子优化基因的氢氧化物。铁氧还蛋白（ferredoxin）基因和铁氧还蛋白-NADP ^{+ 还原酶（铁氧还蛋白-NADP ^{+ ）还原酶提供了用含有该基因的重组表达载体转化的重组微生物。使用重组微生物，成本高昂的细胞生物转化系统，该系统规模的毛发生长促进剂可在以后有效的规模生产出CsA-4-OH，有望在以后的生产中得到有效应用。}}

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公开/公告号KR101803174B1

专利类型
公开/公告日2017-11-29

原文格式PDF
申请/专利权人 인하대학교 산학협력단;칭다오 인스티튜트 오브 바이오에너지 앤드 바이오프로세스 테크놀로지 차이니즈 아카데미 오브 사이언스즈;
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申请/专利号KR20160026269
发明设计人 김응수;쉐잉 리;
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申请日2016-03-04
分类号C12N9/02;C07K7/64;C12N15/52;C12N15/70;C12N9/04;C12P21/02;
国家 KR
入库时间 2022-08-21 12:41:31

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