FILLER FOR AFFINITY CHROMATOGRAPHY AND METHOD FOR ISOLATING IMMUNOGLOBULIN

摘要

The present invention provides a filler for affinity chromatography excellent in alkali resistance and a method for isolating an immunoglobulin. The filler for affinity chromatography of the present invention is a filler in which a protein represented by the following general formula (1) is immobilized on a carrier. Formula 1 (Wherein R represents an amino acid sequence comprising 4 to 300 amino acid residues comprising 4 to 20 histidine consecutive sites, and R ² represents a Z domain of protein A or a fragment thereof or a variant thereof An amino acid sequence capable of binding to an immunoglobulin, wherein R is attached to the C-terminal or N-terminal of R ² , including from 50 to 500 amino acid residues)