Method of mass propagation of bamboo by in vitro culture

摘要

The present invention relates to a method of mass propagating bamboo by using in vitro culture, and a medium composition for in vitro culture of bamboo. More specifically, the present invention relates to a method of mass propagating bamboo by using shoots of a temperate bamboo growing in a temperate zone; and a bamboo plant body produced thereby. The mass propagation method comprises: (a) extracting a node piece including an auxiliary bud from a bamboo branch and sterilizing the same; (b) culturing the sterilized node piece to generate a shoot; (c) sub-culturing the generated shoot to induce multiple shoots; (d) culturing the induced multiple shoots to induce rooting; (e) culturing the rooting-induced multiple shoots to grow roots and stems to thereby obtain a plantlet; and (f) subjecting the plantlet to sensory adaptation to grow the same into a normal bamboo plant body. Accordingly, the method of the present invention can mass propagate identical bamboo entities over a short period of time through in vitro culture, and by inducing rooting, can obtain normal plant bodies whenever the need arises, and thus can be extremely beneficially used in the mass propagation of bamboo.