MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILIARY TREE AND METHODS OF ISOLATING SAME

摘要

The present invention is based on the finding that fetal, neonatal, pediatric, and adult biliary tract tissues and deaths of up to 72 hours after death (preferably within 10 hours after death), respectively, and grow into multiple endoderm tissue comprising liver, biliary and pancreatic tissue , Multipotential cells, multipotent cell groups, and enriched multipotent cell population. Multidisciplinary stem / progenitor cells and cell groups are found in the peribiliary glands, and descendent progenitors from them are present throughout the gallbladder. A large number of bile duct peripapillary lines are found in the biliary system, such as the portal site, the gallbladder duct, the gallbladder duct, the general duct, the total - pancreatic duct, and the gallbladder. Related multipotent cells, multipotent cell populations and descending precursors are found throughout the biliary system, including the gallbladder with no bile duct periplasm. Also included are compositions comprising them, identifying and isolating them, maintaining them in a culture, expanding them in a culture, and administering them in vivo or ex vivo to liver, bile duct or pancreatic fate (such as liver cells, Pancreatic islet cells). Methods of using multipotent cells and / or multipotent cells are also provided.