DNA METHOD AND APPARATUS FOR ULTRASENSITIVE QUANTIFICATION OF DNA BIOMARKER

摘要

The present invention relates to a method and an apparatus for ultrasensitive quantitative analysis of a DNA biomarker, and more specifically, provides a method and a kit for quantitative analysis of target DNA using an atomic force microscope. The method for quantitative analysis of DNA according to the present invention enables the quantitative analysis of target DNA at extremely low concentration, which has been difficult to carry out by a conventional method, through adhesion force-mapping by using an atomic force microscope, without causing target DNA amplification and transformation or requiring the use of a fluorescent marker. In particular, the method applies the size optimization of a probe DNA spot generated to capture target DNA, thereby enabling the quantitative analysis of ten or less target DNA molecules on various substrates. When applied for diagnosing a disease, monitoring the prognosis of treatment, and the like, the method for ultrasensitive quantitative analysis of DNA according to the present invention is expected to be applicable not only to the early diagnosis of a disease but also to accurately monitoring the progress of surgery and treatment.