Culture method for differentiating gonadal cells into functionally mature oocyte cells

摘要

The present invention also provides a method for differentiating a reproductive cell into a functionally mature GV-deficient cell by in vitro culture. The present invention provides a method for producing a follicular ovarian cancer cell, comprising the steps of (a) culturing the germ cells and the supporting cells adjacent to the germ cells under the conditions of eliminating the effect of factors having estrogen or estrogen- A step of partially cleaving the binding between the granulosa cell layer and the caudate cell layer in the ovarian cells, the granulosa cell layer, and the ganglion cell layer constituting the formed secondary follicles; (c) And a method for differentiating gonadal cells into functional GV-gonad cells in vitro, comprising culturing gonadotropin-releasing cells in a medium containing a polymeric compound to divide gonadal cells into functional GV gonadotropic cells .