Regulation of Gene Expression by Utter-Modulated Polyadenylation

摘要

The present invention relates to a polynucleotide construct for the modulation of gene expression by aptamer-based modulation of U1 small nuclear ribonucleoprotein (snRNP) -mediated inhibition of polyadenylation and to the presence or absence of a ligand binding to an aptamer Methods of using constructs to regulate gene expression are provided. Polynucleotide constructs contain a U1 binding site in conjunction with a riboswitch comprising an effector region and an aptamer so that expression of the target gene occurs when the aptamer binds to the ligand.