首页>
外国专利>
Method for generating induced pluripotent stem cells using histone deacetylase inhibitor and bone morphogenetic protein pathway blocker
Method for generating induced pluripotent stem cells using histone deacetylase inhibitor and bone morphogenetic protein pathway blocker
展开▼
机译:使用组蛋白脱乙酰基酶抑制剂和骨形态发生蛋白途径阻断剂产生诱导性多能干细胞的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to a method for producing a recombinant human somatic cell, comprising the steps of: (a) culturing a human-derived somatic cell into which a gene encoding a dediffering inducer is introduced in the presence of at least one selected from the group consisting of a protein kinase C inhibitor, a histone deacetylase inhibitor, Culturing in a medium containing a differentiation-enhancing agent; And (b) separating embryonic stem cell-like colonies from the culture obtained from step (a). The present invention also provides a method for producing iPS cells. The production method of the present invention is capable of producing reprogrammed pluripotent stem cells at high efficiency and also capable of producing reprogrammed pluripotent stem cells under xenopathogen-free and feeder cell-free conditions Can be manufactured.
展开▼