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A Process for Purifying Vitamin K dependent Proteins such as Coagulation Factor
A Process for Purifying Vitamin K dependent Proteins such as Coagulation Factor
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机译:纯化维生素K依赖性蛋白如凝血因子的方法
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摘要
A method for preparing a prion-free vitamin K-dependent protein by a purification sequence using chromatography, At least one chromatography is carried out using a multimodal resin; Providing an aqueous solution of a fraction comprising a vitamin K dependent protein in an aqueous solution; Contacting a fraction comprising a vitamin K dependent protein with a multimodal resin at a pH of from 6 to 9; - optionally, washing the multimodal resin with the adsorbed vitamin K dependent protein to remove contaminants and maintain the vitamin K dependent protein, prior to eluting the vitamin K dependent protein, with an aqueous wash buffer; eluting the vitamin K dependent protein from the multimodal resin with a buffer comprising arginine at pH 6-9; And Optionally collecting the fraction containing the vitamin K dependent protein in a purified or enriched form; / RTI
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