3 A method of Effects of three dimensional culture of spermatogonial stem cell and use of the same

摘要

The present invention relates to a three-dimensional in vitro culture method for spermatogonial stem cells, and more particularly to a method for culturing spermatogonial stem cells in a three-dimensional hydrogel based on an agarose-based three-dimensional hydrogel or a polyethylene glycol (PEG) To maintain self regeneration and undifferentiation of porcine spermatogonial stem cells and their use. As can be seen through the present invention, agarose and PEG-based 3D hydrogels can be used as powerful systems for the maintenance of spermatogonial stem cells.;