Method for using nanoparticles as nucleation agents for the crystallization of proteins

摘要

The present invention relates to a protein crystallization method using nanoparticles as a nucleation inducer, and more particularly, to a method for crystallizing a protein using nanoparticles as a nucleation-inducing agent by using gold nanoparticles of various sizes and shapes having carboxyl groups,Bacillus subtilisYesR, chicken egg white lysozyme, bovine serum albumin,Alicyclobacillus acidocaldariusacetyl-CoA carboxylase,Listeria monocytogeneshypothetical protein protein and Ni²⁺As a result of performing the crystallization under the ionic condition, it was confirmed that when the crystallization was performed using gold nanoparticles, the success rate of crystallization was increased and various crystallization conditions could be found, compared with the control without gold nanoparticles, Formation induction method can be usefully used for protein structure identification by increasing protein crystallization success rate.;