Tissue culture method for mass proliferation of walnuts

摘要

The present invention relates to a tissue culture method for mass proliferation of walnuts which is composed of disinfection of collected walnut seed trees and internode culture of a growth point, growth culture, rooting pretreatment culture, rooting culture, first and second purifications and field transplantation. Also, the present invention provides a growth condition enabling optimum growth efficiency and mass production in a short time such as the number of roots, a rooting rate and a root length throughout the overall tissue culture.