Method of vitrification of ovarian tissue

• 1. 一种提高卵巢组织玻璃化冷冻效率的方法 [P] . 中国专利： CN111838131B . 2021.09.24
• 2. 一种提高卵巢组织玻璃化冷冻效率的方法 [P] . 中国专利： CN111838131A . 2020-10-30
• 3. Method of vitrification of ovarian tissue [P] . 俄罗斯专利： RU2016148995A . 2018-06-18

  机译： 卵巢组织玻璃化的方法

• 4. METHOD vitrification of ovarian tissue [P] . 俄罗斯专利： RU2012103059A . 2013-08-10

  机译： 卵巢组织玻璃化的方法

• 5. TOOL FOR VITRIFICATION PRESERVATION OF COLLECTED TISSUE PIECE, PLATE FOR FREEZE-PRESERVATION OF TISSUE PIECE AND METHOD FOR VITRIFICATION PRESERVATION OF COLLECTED TISSUE PIECE [P] . 日本专利： JP2008222640A . 2008-09-25

  机译： 收集的组织切片的玻璃化保存工具，组织切片的冷冻保存板和组织的切片的保存玻璃化方法

• 1. Prevention and treatment of iatrogenic premature ovarian insufficiency:interpretation of the first Chinese guideline on ovarian tissue cryopreservation and transplantation [J] . Xiangyan Ruan, Jiaojiao Cheng, Juan Du, 全球健康杂志（英文） . 2021,第002期
• 2. Prevention and treatment of iatrogenic premature ovarian insufficiency:r interpretation of the first Chinese guideline on ovarian tissue cryopreservation and transplantation [J] . Xiangyan Ruan, Jiaojiao Cheng, Juan Du, 全球健康杂志（英文） . 2021,第002期
• 3. Comparative Study on Antioxidative System in Normal and Vitrified Shoots of Populus suaveolens in Tissue Culture [J] . Lin Shanzhi, Zhang Zhiyi, Lin Yuanzhen, 中国林学（英文版） . 2004,第003期
• 4. Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods [J] . R. R. Santos, T. Tharasanit, T. Van Haeften, Cell and Tissue Research . 2007,第1期
• 5. Optimal vitrification protocol for mouse ovarian tissue cryopreservation: Effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival [J] . YoumH.W., LeeJ.R., LeeJ., Human Reproduction . 2014,第4期
• 6. Vitrification of Bovine Ovarian Tissue by the Solid-Surface Vitrification Method [J] . Juliana J.H. Celestino, Regiane Rodrigues dos Santos, Monica A.P. Melo, Biopreservation and biobanking . 2010,第4期
• 7. The Profile of Ovarian Tissue After Vitrification Using Ethylene Glycol (EG) and Longan Honey in Female Sprague-Dawley Rats (Rattus norvegicus L.) [C] . I. Muhiardi, N. Anita, A. Abinawanto, International Symposium on Current Progress in Mathematics and Sciences . 2019
• 8. Equine preantral follicles: Ultrasound-guided biopsy pick-up method to harvest ovarian tissue for in vitro processing, evaluation and culture [D] . Haag, Keith T. 2012
• 9. Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method [O] . Ta-Chin Lin, Jui-Mei Yen, Tsung-Cheng Kuo, 2008
• 10. Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method [O] . Ta-Chin Lin, Jui-mei Yen, Tsung-Cheng Kuo, 2008

• 1. Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa [J] . Masindi L. Mphaphathi ,Dibungi Luseba ,Ben Sutherland . 动物科学期刊（英文） . 2012,第3期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. 人类卵巢组织玻璃化冷冻保存新方法的建立 [C] . 李宇彬 ,周灿权 ,杨国奋 . 2006中国医师协会国际生殖系统疾病学术大会 . 2006
• 4. 三种方法应用于人卵巢组织玻璃化冷冻的效果分析 [A] . 闫惠惠 . 2015