FIELD: biotechnology.SUBSTANCE: disclosed is a method of producing brucell biomass of vaccine strains. Method involves deep cultivation of the brucella culture on a liquid nutrient medium minimized by content of the protein component under conditions of regulation of partial pressure dissolved in the medium of oxygen and dosed supply of glucose according to the scheme: introduction of 10.0 g/l of glucose into the nutrient medium before seeding, then continuous dosing thereof at 1.5 g/l per hour from 16 to 19 hours of growth and 2.0 g/l per hour from 20 to 24 hours of growth.EFFECT: method provides higher yield of Brucella biomass.1 cl, 3 tbl
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机译:技术领域:生物技术。公开了一种生产疫苗株的布鲁氏菌生物质的方法。该方法包括在液体营养培养基上进行布鲁氏菌培养物的深层培养,并根据以下方案调节溶解在氧气介质中的分压并按一定剂量供应葡萄糖,从而通过蛋白质成分的含量使其降至最低限度:引入10.0 g / l在播种前将葡萄糖注入营养培养基中,然后在生长16至19小时以每小时1.5 g / l的速度连续添加,在生长20至24小时以每小时2.0 g / l的速度连续施用。效果:该方法可提供更高的布鲁氏菌生物量产量.1厘升,3汤匙
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