METHOD FOR ARTIFICIAL TOOTH BUD PRODUCTION IN VITRO AND OBTAINED ARTIFICIAL TOOTH BUD

摘要

FIELD: medicine.;SUBSTANCE: method for production of an artificial tooth bud in vitro involves: a) obtaining of selected mesenchymal cells of the dental pulp; b) dental pulp mesenchymal cells culturing in a monolayer on a surface for adhesive cells; and c) dental pulp mesenchymal cells culturing under non-adhesive conditions in culture vessels with culture surface with ultra-low attachment of cells to form a cellular unit, which is an artificial tooth bud. An artificial tooth bud obtained by the above method, a transplant containing such artificial tooth bud and a pharmaceutical composition for treatment of tooth fracture and/or damage or tooth loss, containing the above artificial tooth bud or transplant, and at least one pharmaceutically acceptable excipient is proposed as well. In addition, a method for in vitro screening of substances which modulate the dental tissue properties comprising the steps: obtaining of a sample of the above artificial tooth bud, or cellular unit, or tissue derived from it; sample division into pieces; incubation of at least one part with the substance subject to screening, and comparison of parameters measured for the treated part with another part, which is not incubated with the substance subject to screening.;EFFECT: method allows to produce an artificial tooth bud from adult cells to eliminate the need for embryonic cells or tissues, and only one cell type is required, namely the selected tooth pulp mesenchymal cells, or two cell types, namely the selected tooth pulp mesenchymal cells and keratinocytes; without application of any artificial biological or nonbiological frame or media that do not come from cells used in the production of the specified artificial tooth bud.;15 cl, 1 ex, 16 dwg