SYSTEM FOR ADDRESS CONTROL OF BRAIN NEURONS OF LIVING FREE-MOVING ANIMALS BASED ON FROZEN FIBER-OPTICAL PROBE WITH MULTI-CHANNEL FIBERS

摘要

FIELD: medicine.;SUBSTANCE: system includes a laser excitation system, an optical response recording system, an openable optical fiber probe (OFP) based on multichannel optical fiber (MOF) with micron-sized lightguides, containing a module mounted on the animal's skull and a mating openable part providing its communication with optical excitation systems and recording of the optical response, in accordance with what is set forth in the claims. The OFP consists of two parts, the first made with a possibility of fixation on the animal's skull and contains a ceramic cylindrical ferrule with a short MOF section attached thereto. The second part of the probe contains a connector with another cylindrical ceramic ferrule and a long MOF section attached thereto. The second part of the probe is connected to a laser optical excitation system and a recording and monitoring system. The MOF sections contain at least one thousand coaxially located micron-size lightguides. The distal end of the first optical fiber section has a flat surface oblique at an angle of 30 to 60 degrees relative to the MOF axis, and MOF diameter is from 250 mcm to 600 mcm. When the method is implemented, laser radiation of a selected wavelength and power is generated, allowing to reach the photo activation threshold of cells containing genetically embedded photosensitive proteins. The laser radiation is focused by means of a scanning system and a focusing module into the selected lightguides of the long section of MOF located inside the connector in optical contact with the short MOF section attached to the animal's skull so that its distal end is located in the selected region of the animal's brain. The radiation generates a measurable fluorescence signal, it is transmitted through a probe to the optical recording and monitoring system with a computer, where the signals obtained are subjected to the appropriate analysis.;EFFECT: group of inventions allows to obtain information on functioning of the individual brain cells and provide addressable influence thereon by a long-term series of experiments on the same animal in any brain structure, while providing reception of an optical signal from one and the same group of cells.;6 cl, 4 dwg