METHOD FOR DETERMINATION OF METHYLDOPE CONCENTRATION IN HUMAN BLOOD PLASMA

摘要

FIELD: pharmacology.;SUBSTANCE: method for determination of methyldope concentration in the human plasma includes sampling of an aliquot of plasma, its deproteinization, the obtained mixture is centrifuged, methyldope concentration is measured in the supernatant by high-performance liquid chromatography with tandem mass spectrometry in the range from 0.020 to 3 mcg/ml by a calibration graph; the difference is in the application of a deuterated internal methyldope standard solution in methanol for plasma deproteinization, chromatographic separation of the matrix components uses two chromatographic columns Phenomenex Luna Phenyl-Hexyl (50×3.0 mm, 5 mcm) and Phenomenex Synergi Fusion-RP 80A (150×4.6 mm, 4 mcm), and the electrospray ionization is performed simultaneously with chemical ionization at atmospheric pressure.;EFFECT: improved method.;1 ex, 5 tbl