SET OF PRIMERS SEQUENCE AND ALLELE-SPECIFIC PROBES FOR SIMULTANEOUS FOUR MUTANT KAPPA-CASEIN ALLELES GENE DIAGNOSTICS IN BOVINE CATTLE

摘要

FIELD: veterinary medicine.;SUBSTANCE: invention can be used in veterinary practice and zootechnics to diagnose kappa-casein four important alleles (κ-CN^A, κ-CN^B, κ-CN^C, κ-CN^E). Proposed is a set of primers sequences and allele-specific probes for simultaneous four mutant kappa-casein alleles gene diagnostics in cattle by performing of the real time polymerase chain reactions, having the following structure: CSN3_F tgtgctgagtaggtatcctagttatgg; CSN3_R gcgttgtcttctttgatgtctccttag; CSN3-HinfI-wt2 (FAM)-tagctactctagaagattctccaga-(RTQ1); CSN3-HinfI-m2 (R6G)-gctactctagaagcttctccagaa-(BHQ2); CSN3-HaeIII-wt (FAM)-agaagttattgagAgcccacct-(RTQ1); CSN3-HaeIII-m2 (R6G)-cagaagttattgagGgccc-(BHQ2); CSN3-MaeII-wt (FAM)-catggcacGtcacccaca-(RTQ1); CSN3-MaeII-m (R6G)-tggcacAtcacccacacc-(BHQ2).;EFFECT: simultaneous four mutant kappa-casein alleles gene diagnostics in cattle.;1 cl, 1 dwg, 4 tbl