METHOD OF EXPRESS DIAGNOSTICS OF NODULAR DERMATITIS IN CATTLE

摘要

FIELD: veterinary.;SUBSTANCE: invention relates to the field of veterinary virology, in particular to the method of express diagnostics of nodular dermatitis in cattle. Method of express diagnostics of the nodular dermatitis virus of cattle includes the sampling of pathological material from the source of infectious disease from all animals, located in the focus of an infectious disease, and rapid diagnosis of the obtained pathological material during the day using the PCR method with fluorescent recognition of results in real time using a thermal cycler of the RotorGene type to identify carriers of the virus of nodular dermatitis at the initial stage of infection, PCR results are taken into account by the presence or absence of the intersection of the fluorescence curve with the threshold line established at the appropriate level, if the growth of a specific signal is observed, then the sample is considered positive - the nodular dermatitis virus of cattle is present, while the values of the control samples are within the limits of the norm, if the growth of a specific signal is not observed, then the sample is considered negative - the nodular dermatitis virus of large cattle is absent, and the values of the control samples are also within the normal range.;EFFECT: express diagnostics of nodular dermatitis in cattle.;1 cl, 3 tbl, 1 dwg