METHOD FOR DETECTING DISORDERS IN CHILDREN OF IMMUNOLOGICAL REACTIVITY IN CONDITIONS OF EXCESSIVE EXPOSURE TO STRONTIUM

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine, namely to pediatrics and immunology, and can be used to detect impairment in children of immunological reactivity in conditions of excessive exposure to strontium. For this purpose, blood samples are taken from the child with determination of the strontium content in it, as well as samples of buccal epithelium with the release of deoxyribonucleic acid (DNA). Then, genotyping of the polymorphism of the TLR4 and HLA-DR genes is carried out on a detecting polymerase chain reaction (PCR) amplifier in real time, using as a primer a DNA site by examining the genotypes of the TLR4 gene A8595G (rs1927911) and the HLA-DR gene C/T (rs3135388), while establishing for each of these genes one of the following states: heterozygous, or normal homozygous, or variant homozygous. Under simultaneous fulfillment of the following conditions: the presence of variant homozygous or heterozygous genotypes of the gene TLR4 A8595G (rs1927911) and/or the HLA gene-DR C/T (rs3135388) and exceeding the concentration of strontium in blood above the reference level by 20 %, the child is diagnosed with immunological reactivity disorders in conditions of excessive exposure to strontium.;EFFECT: method allows to provide an accurate estimate of the effect of strontium on the presence of a violation of immunological reactivity, with the possibility of subsequently judging the development of autoimmune and atopic states in children already at the early stages of their formation.;1 cl, 1 tbl, 2 ex