RECOMBINANT PLASMID DNA pET15/N-Dest+ AND METHOD FOR THE PREPARATION, THE STRAIN OF ESCHERICHIA COLI BL21(DE3)GOLD, TRANSFORMED BY RECOMBINANT DNA pET15/N-Dest+, AND A METHOD OF ITS RECEPTION, A METHOD FOR OBTAINING RECOMBINANT BIFUNCTION ENZYME - DESTABILASE OF MEDICAL LEECH

摘要

FIELD: biochemistry; biotechnology.;SUBSTANCE: invention relates to the field of biochemistry and biotechnology, in particular to recombinant plasmid DNA pET15/N-Dest+. Plasmid DNA is intended for expression in the Escherichia coli cells of the destabilase gene of the medicinal leech Hirudo medicinalis. Invention also concerns a method of producing plasmid DNA pET15/N-Dest+ and to the strain Escherichia coli BL21(DE3)Gold/pET15/N-Dest+, obtained by transformation of the strain Escherichia coli BL21(DE3)Gold of plasmid DNA pET15/N-Dest+. Strain is a producer of recombinant destabilase. Invention also relates to a method of producing strain Escherichia coli BL21(DE3)Gold/pET15/N-Dest+ and a method for producing recombinant destabilase using the above strain.;EFFECT: invention makes it possible to obtain recombinant destabilase with high yield and purity.;10 cl, 4 dwg, 2 tbl, 6 ex