METHOD OF CULTIVATION OF MICROALGAE COELASTRELLA RUBESCENS FOR PRODUCTION OF CAROTENOIDS AND LIPIDS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to microalgae biotechnology and can be used to obtain carotenoids and lipids. Method of cultivation of microalgae Coelastrella rubescens for the simultaneous production of ketocarotenoids of the astaxanthin group and lipids for production of biodiesel. Method provides for culturing Coelastrella rubescens method of a two-stage storage culture on a nutrient medium with observance at the I (“green”) stage of the illumination regime of 15 h light/9 h dark. Before cultivation on the II (“red”) stage on the medium BBM, the culture is subjected to a complex stress action – dilution of the obtained biomass with nutrient medium BBM, reduced in nitrogen and phosphorus, and switch to a 24-hour lighting mode. At the I (“green”) stage C. rubescens are cultured for 11 days on the MBBM nutrient medium at n_st=1.1–1.3 cells⋅ml^-1 and pH 7, observing the conditions of cultivation: I-140 mcE⋅m^-2⋅s^-1, T – 25–26 °C, air blowing speed – 1 l⋅min^-1⋅l^-1. At the II (“red”) stage of cultivation, 11-fold dilution of the culture is performed, which is then cultivated for 8 days.;EFFECT: invention provides an increase in average daily carotenoid productivity of the culture by 53 % and reduction in cultivation period from 27 to 19 days.;1 cl, 3 dwg, 3 tbl, 1 ex