A process for the qualitative assessment of a flow cytometric analysis, and a reagent for carrying out the method

摘要

The invention proposes a process for the qualitative assessment of a through flow cytometer's analysis, whereby a quantitative determination of cells by means of a flow cytometer takes place, the flow cytometer transmits light of a specific wavelength, and a on this light is tuned to the fluorescence - dye is selected, a blood sample monoclonal, with the selected fluorescence - dye antibodies are added, which rest against epitopes of the cells present in the blood sample, the blood sample containing the antibodies in the flow cytometer with the light and is acted on by the cd4 - cells of the emitted fluorescent light is detected by means of a detector, and on the basis of the detector signals the number or the quantity of the contained in the blood sample cd4 - cells is determined, and wherein the blood sample which is designated as beads, likewise provided with a fluorescence - dye subsequently be added before they can be separated in the flow cytometer with the light is applied; the beads are used, the light - emission behaviors are derived from the light - emission behaviors of the antibody in the manner of differing from that in the detector different the beads and the antibodies characteristic detector signals, and the detector signals are evaluated in the manner that, on the basis of the detector signals caused by the beads is determined, the state of the flow cytometer. Furthermore, the present invention describes a reagent for carrying out the method, wherein the reagent monoclonal, which is provided with a fluorescence - dye contains antibody which is referred to as well as beads, likewise provided with a fluorescence - dye subsequently.