首页> 外国专利> CULTURE CONTAINER FOR ADHERENT CELLS AND METHOD FOR PRODUCING CULTURE CONTAINER FOR ADHERENT CELLS

CULTURE CONTAINER FOR ADHERENT CELLS AND METHOD FOR PRODUCING CULTURE CONTAINER FOR ADHERENT CELLS

机译:用于贴壁细胞的培养容器和制备用于贴壁细胞的培养容器的方法

摘要

A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95° or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality "advancing contact angle - receding contact angle 25°" when water runs down along the inner surface. It is preferable that the polyolefin include an antioxidant (particularly preferably a phenol-based antioxidant). An adherent cell culture vessel that has a culture surface having a contact angle and a hysteresis within the above ranges may be obtained by applying UV rays having a wavelength other than an ozone-generating wavelength to a polyolefin.
机译:提供了用于培养贴壁细胞的培养袋,不需要高度清洁的生产环境和复杂的生产步骤,例如掩盖步骤。由聚烯烃形成的贴壁细胞培养容器被配置为使得培养容器的内表面的一部分或全部具有95°以上的静态水接触角,并且具有前进接触角和后退接触角。当水沿着内表面流下时,满足不等式“前进接触角-后退接触角> 25°”的不等式。聚烯烃优选包括抗氧化剂(特别优选基于酚的抗氧化剂)。可以通过将具有不同于产生臭氧的波长的波长的紫外线施加到聚烯烃上来获得具有在上述范围内的接触角和滞后性的培养表面的贴壁细胞培养容器。

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