SYNTHETIC GENE FOR EXPRESSING SM-14 IN PICHIA PASTORIS, METHODS FOR PRODUCING AND PURIFYING SM-14 AND THE USE THEREOF AS A VACCINE AND DIAGNOSTIC MEDIUM

摘要

The present invention is related to the field of recombinant protein production using a synthetic gene associated with high protein expression in Pichia pastoris. More specifically, this invention describes the production of Sm14 Schistosoma mansoni recombinant protein. A synthetic gene was created to promote high Sm14 expression, and with this gene we obtained and genetically manipulated a Pichia pastoris strain for effectively producing a vaccine. We have also improved that protein's production and purification processes from P. pastoris cells; the industrial production of such processes may be scheduled.