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MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS
MELTING CURVE ANALYSIS USING PNA PROBE, METHOD AND KIT FOR ANALYZING NUCLEOTIDE POLYMORPHISM USING MELTING CURVE ANALYSIS
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机译:使用PNA探针进行熔解曲线分析,方法和试剂盒,用于通过熔解曲线分析来分析核苷酸多态性
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摘要
Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.
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