MAGNETIC BEADS-BASED ELECTROCHEMICAL BIOSENSOR

摘要

The present invention is generally directed to methods for detecting and/or quantifying miRNA, RNA or DNA molecules of interest in at least one isolated biological sample. Basically the general procedure followed by the methods of the present invention involve four main steps: (i) homogeneous hybridization of the synthetic DNA or RNA probe and the target miRNA, RNA or DNA molecule of interest; (ii) capture of the resultant DNA/RNA, DNA/DNA or RNA/RNA duplex by the antibody-modified magnetic particles, preferably MBs, in solution; (iii) enzymatic labeling of the biotinylated DNA/RNA, DNA/DNA or RNA/RNA duplex captured onto the antibody-modified magnetic particles, preferably MBs and (iv) electrochemical detection of the modified-magnetic particles on an electrochemical sensor, preferably on screen-printed carbon electrodes (SPCEs).