METHOD OF IDENTIFICATION OF EUROPEAN FRESHWATER FISH AND HYBRIDES IN BIOLOGICAL MATERIALS BY S7ICAPS METHOD

摘要

A method of identification of European freshwater fish species/lineages in biological materials and fish products using a method based on amplification of intron 1 of the gene encoding the S7 ribosomal protein RPS7 with primers which bind internally in the intron and in which the amplicons can be distinguished by length or by a specific restriction pattern of partial genomic sequence of the first intron of the S7 gene. The method consists in using of genus or group-specific primer sets, unique combination design of variable InDel-CAPS and/or SNP-CAPS markers and is qualitative.